diff --git a/CHANGES/3480.bugfix b/CHANGES/3480.bugfix
new file mode 100644
index 00000000000..eea52e84135
--- /dev/null
+++ b/CHANGES/3480.bugfix
@@ -0,0 +1 @@
+Replace ``collections.MutableMapping`` with ``collections.abc.MutableMapping`` to avoid a deprecation warning.
diff --git a/aiohttp/web_response.py b/aiohttp/web_response.py
index ba90d937243..750e040afbc 100644
--- a/aiohttp/web_response.py
+++ b/aiohttp/web_response.py
@@ -1,5 +1,5 @@
 import asyncio  # noqa
-import collections  # noqa
+import collections.abc  # noqa
 import datetime
 import enum
 import json
@@ -39,7 +39,7 @@
     from .web_request import BaseRequest  # noqa
     BaseClass = MutableMapping[str, Any]
 else:
-    BaseClass = collections.MutableMapping
+    BaseClass = collections.abc.MutableMapping
 
 
 class ContentCoding(enum.Enum):
diff --git a/tests/test_web_response.py b/tests/test_web_response.py
index 42ccfe71dbb..70eb35b769d 100644
--- a/tests/test_web_response.py
+++ b/tests/test_web_response.py
@@ -1,4 +1,4 @@
-import collections
+import collections.abc
 import datetime
 import gzip
 import json
@@ -94,7 +94,7 @@ def test_stream_response_eq() -> None:
 
 def test_stream_response_is_mutable_mapping() -> None:
     resp = StreamResponse()
-    assert isinstance(resp, collections.MutableMapping)
+    assert isinstance(resp, collections.abc.MutableMapping)
     resp['key'] = 'value'
     assert 'value' == resp['key']