diff --git a/packages/aws-cdk-lib/aws-lambda/lib/function.ts b/packages/aws-cdk-lib/aws-lambda/lib/function.ts
index eebe0b37db0ba..2f804ca0d4f06 100644
--- a/packages/aws-cdk-lib/aws-lambda/lib/function.ts
+++ b/packages/aws-cdk-lib/aws-lambda/lib/function.ts
@@ -916,6 +916,14 @@ export class Function extends FunctionBase {
     // add additional managed policies when necessary
     if (props.filesystem) {
       const config = props.filesystem.config;
+      if (!Token.isUnresolved(config.localMountPath)) {
+        if (!/^\/mnt\/[a-zA-Z0-9-_.]+$/.test(config.localMountPath)) {
+          throw new Error(`Local mount path should match with ^/mnt/[a-zA-Z0-9-_.]+$ but given ${config.localMountPath}.`);
+        }
+        if (config.localMountPath.length > 160) {
+          throw new Error(`Local mount path can not be longer than 160 characters but has ${config.localMountPath.length} characters.`);
+        }
+      }
       if (config.policies) {
         config.policies.forEach(p => {
           this.role?.addToPrincipalPolicy(p);
diff --git a/packages/aws-cdk-lib/aws-lambda/test/function.test.ts b/packages/aws-cdk-lib/aws-lambda/test/function.test.ts
index c5228bb2cd82c..a6d982ddf5bdf 100644
--- a/packages/aws-cdk-lib/aws-lambda/test/function.test.ts
+++ b/packages/aws-cdk-lib/aws-lambda/test/function.test.ts
@@ -2923,6 +2923,99 @@ describe('function', () => {
       });
     });
 
+    test('validate localMountPath format when mounting efs', () => {
+      // GIVEN
+      const stack = new cdk.Stack();
+      const vpc = new ec2.Vpc(stack, 'Vpc', {
+        maxAzs: 3,
+        natGateways: 1,
+      });
+      const securityGroup = new ec2.SecurityGroup(stack, 'LambdaSG', {
+        vpc,
+        allowAllOutbound: false,
+      });
+
+      const fs = new efs.FileSystem(stack, 'Efs', {
+        vpc,
+      });
+      const accessPoint = fs.addAccessPoint('AccessPoint');
+
+      // THEN
+      expect(() => {
+        new lambda.Function(stack, 'MyFunction', {
+          vpc,
+          handler: 'foo',
+          securityGroups: [securityGroup],
+          runtime: lambda.Runtime.NODEJS_LATEST,
+          code: lambda.Code.fromAsset(path.join(__dirname, 'handler.zip')),
+          filesystem: lambda.FileSystem.fromEfsAccessPoint(accessPoint, '/not-mnt/foo-bar'),
+        });
+      }).toThrow('Local mount path should match with ^/mnt/[a-zA-Z0-9-_.]+$ but given /not-mnt/foo-bar');
+    });
+
+    test('validate localMountPath length when mounting efs', () => {
+      // GIVEN
+      const stack = new cdk.Stack();
+      const vpc = new ec2.Vpc(stack, 'Vpc', {
+        maxAzs: 3,
+        natGateways: 1,
+      });
+      const securityGroup = new ec2.SecurityGroup(stack, 'LambdaSG', {
+        vpc,
+        allowAllOutbound: false,
+      });
+
+      const fs = new efs.FileSystem(stack, 'Efs', {
+        vpc,
+      });
+      const accessPoint = fs.addAccessPoint('AccessPoint');
+
+      // THEN
+      expect(() => {
+        new lambda.Function(stack, 'MyFunction', {
+          vpc,
+          handler: 'foo',
+          securityGroups: [securityGroup],
+          runtime: lambda.Runtime.NODEJS_LATEST,
+          code: lambda.Code.fromAsset(path.join(__dirname, 'handler.zip')),
+          filesystem: lambda.FileSystem.fromEfsAccessPoint(accessPoint, `/mnt/${'a'.repeat(160)}`),
+        });
+      }).toThrow('Local mount path can not be longer than 160 characters but has 165 characters');
+    });
+
+    test('No error when local mount path is Tokenized and Unresolved', () => {
+      // GIVEN
+      const realLocalMountPath = '/not-mnt/foo-bar';
+      const tokenizedLocalMountPath = cdk.Token.asString(new cdk.Intrinsic(realLocalMountPath));
+
+      const stack = new cdk.Stack();
+      const vpc = new ec2.Vpc(stack, 'Vpc', {
+        maxAzs: 3,
+        natGateways: 1,
+      });
+      const securityGroup = new ec2.SecurityGroup(stack, 'LambdaSG', {
+        vpc,
+        allowAllOutbound: false,
+      });
+
+      const fs = new efs.FileSystem(stack, 'Efs', {
+        vpc,
+      });
+      const accessPoint = fs.addAccessPoint('AccessPoint');
+
+      // THEN
+      expect(() => {
+        new lambda.Function(stack, 'MyFunction', {
+          vpc,
+          handler: 'foo',
+          securityGroups: [securityGroup],
+          runtime: lambda.Runtime.NODEJS_LATEST,
+          code: lambda.Code.fromAsset(path.join(__dirname, 'handler.zip')),
+          filesystem: lambda.FileSystem.fromEfsAccessPoint(accessPoint, tokenizedLocalMountPath),
+        });
+      }).not.toThrow();
+    });
+
     test('correct security group is created when deployed in separate stacks', () => {
       const app = new cdk.App();