diff --git a/CHANGELOG.md b/CHANGELOG.md
index f40b72c..229fc0c 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -1,6 +1,14 @@
 Changelog
 =========
 
+## 3.0.1 (2022-03-24)
+
+### Bug fixes
+
+* Ensure RemoveProjectRoots works for assemblies that are run on a OS that differs from their build OS.
+  | [sgtfrankieboy](https://github.com/sgtfrankieboy)
+  | [#154](https://github.com/bugsnag/bugsnag-dotnet/pull/154)
+
 ## 3.0.0 (2022-01-31)
 
 ### Breaking Changes
diff --git a/Directory.build.props b/Directory.build.props
index 7ab7fcf..70fb10b 100644
--- a/Directory.build.props
+++ b/Directory.build.props
@@ -1,7 +1,5 @@
 <Project>
   <PropertyGroup>
     <OutputPath>$(BaseOutputPath)</OutputPath>
-	<AssemblyOriginatorKeyFile>$(MSBuildThisFileDirectory)Bugsnag.snk</AssemblyOriginatorKeyFile>
-    <SignAssembly>true</SignAssembly>
   </PropertyGroup>
 </Project>
diff --git a/src/Bugsnag/Middleware.cs b/src/Bugsnag/Middleware.cs
index ca20cad..76511b4 100644
--- a/src/Bugsnag/Middleware.cs
+++ b/src/Bugsnag/Middleware.cs
@@ -39,11 +39,26 @@ public static class InternalMiddleware
       {
         var projectRoots = report.Configuration.ProjectRoots.Select(prefix => {
           // if the file prefix is missing a final directory seperator then we should
-          // add one first
-          if (prefix[prefix.Length - 1] != System.IO.Path.DirectorySeparatorChar)
+          // add one first. 
+          var finalChar = prefix[prefix.Length - 1];
+
+          // Check if a prefix is a Unix-based path, if it is we add a `/`.
+          // Otherwise its a Windows-based path and we add `\` instead, if necessary. 
+          if (prefix[0] == '/')
+          {
+            if (finalChar != '/')
+            {
+              prefix = $"{prefix}/";
+            }
+          }
+          else if (finalChar != '\\')
           {
-            prefix = $"{prefix}{System.IO.Path.DirectorySeparatorChar}";
+            // DirectorySeparatorChar is not being used because its `/` on Unix
+            // systems and will break the check when running assemblies build
+            // on Windows but are run on Linux.
+            prefix = $"{prefix}\\";
           }
+
           return prefix;
         }).ToArray();
 
diff --git a/src/Directory.build.props b/src/Directory.build.props
index 4f70f1f..89f0816 100644
--- a/src/Directory.build.props
+++ b/src/Directory.build.props
@@ -1,7 +1,4 @@
 <Project>
-
-  <Import Project="..\Directory.Build.props"/>
-
   <PropertyGroup>
     <Authors>snmaynard kattrali martin308</Authors>
     <Version></Version>
@@ -15,5 +12,7 @@
     <LangVersion>7.1</LangVersion>
     <WarningsAsErrors />
     <NoWarn>1591</NoWarn>
+	<AssemblyOriginatorKeyFile>$(MSBuildStartupDirectory)\Bugsnag.snk</AssemblyOriginatorKeyFile>
+    <SignAssembly>true</SignAssembly>
   </PropertyGroup>
 </Project>
diff --git a/tests/Bugsnag.Tests/MiddlewareTests.cs b/tests/Bugsnag.Tests/MiddlewareTests.cs
index bd875d5..3dbfca8 100644
--- a/tests/Bugsnag.Tests/MiddlewareTests.cs
+++ b/tests/Bugsnag.Tests/MiddlewareTests.cs
@@ -58,8 +58,14 @@ public void ProjectRootStrippingTests(string[] projectRoots, string fileName, st
 
     public static IEnumerable<object[]> ProjectRootTestData()
     {
+      // Tests for Windows-based paths
       yield return new object[] { new string[] { @"C:\app" }, @"C:\app\Class.cs", "Class.cs" };
       yield return new object[] { new string[] { @"C:\app\" }, @"C:\app\Class.cs", "Class.cs" };
+
+      // Tests for Unix-based paths
+      yield return new object[] { new string[] { @"/app" }, @"/app/Class.cs", "Class.cs" };
+      yield return new object[] { new string[] { @"/app/" }, @"/app/Class.cs", "Class.cs" };
+
       // for this scenario we should only strip the file path once, here we
       // have a setup where the first prefix will then also cause the second
       // prefix to match. This should only strip the first prefix
diff --git a/tests/Directory.build.props b/tests/Directory.build.props
index f052b59..fca93c5 100644
--- a/tests/Directory.build.props
+++ b/tests/Directory.build.props
@@ -1,4 +1,5 @@
 <Project>
+  <Import Project="..\Directory.Build.props"/>
   <PropertyGroup>
     <SignAssembly>false</SignAssembly>
   </PropertyGroup>