krakenuniq build (with eukaryotes) errors

[mariafpv]

Hello,

I am having difficulties building my database that includes eukaryotes. I have reviewed several issues and tried to implement the solutions suggested. However, I am still having errors:

Kraken build set to minimize disk writes.
Found 56880 sequence files (*.{fna,fa,ffn,fasta,fsa}) in the library directory.
Creating k-mer set (step 1 of 6)...
Using /hpcfs/home/ciencias_biologicas/mf.penav1/.miniforge/envs/krakenuniq/bin/jellyfish
K-mer set created. [4h56m25.561s]
Skipping step 2, no database reduction requested.
Sorting k-mer set (step 3 of 6)...
db_sort: Getting database into memory ...db_sort: unable to mmap database.jdb: Invalid argument

Below I describe what I have done and I would appreciate if you could help me (I am new to this topic):

I installed krakenuniq it in a cluster (NAME="CentOS Linux", VERSION="8", PLATFORM_ID="platform:el8") and downloaded the taxonomy and the sequences:

krakenuniq-download --db /path/dbfolder refseq/protozoa/Complete_Genome
krakenuniq-download --db /path/dbfolder refseq/fungi/Complete_Genome
krakenuniq-download --db /path/dbfolder refseq/invertebrate/Complete_Genome
krakenuniq-download --db /path/dbfolder refseq/plant/Complete_Genome
krakenuniq-download --db /path/dbfolder refseq/vertebrate_mammalian/Complete_Genome
krakenuniq-download --db /path/dbfolder refseq/vertebrate_other/Complete_Genome

In the indicated folder there were 3 folders and files:
library
library-files.txt
taxonomy

I then ran:

krakenuniq-build --db /path/dbfolder \
    --jellyfish-bin /path/.miniforge/envs/krakenuniq/bin/jellyfish \
    --jellyfish-hash-size 10000000000 \
    --kmer-len 31 \
    --threads 40 \
    --taxids-for-genomes \
    --taxids-for-sequences