diff --git a/includes/Core/User/Audience_Settings.php b/includes/Core/User/Audience_Settings.php
index 56bf47bd390..e201943af1f 100644
--- a/includes/Core/User/Audience_Settings.php
+++ b/includes/Core/User/Audience_Settings.php
@@ -89,6 +89,17 @@ function ( $value ) {
 		return $this->set( array_merge( $settings, $updated ) );
 	}
 
+	/**
+	 * Reset the setting to their default values.
+	 *
+	 * @since n.e.x.t
+	 *
+	 * @return bool True on success, false on failure.
+	 */
+	public function reset() {
+		return $this->set( $this->get_default() );
+	}
+
 	/**
 	 * Gets the callback for sanitizing the setting's value before saving.
 	 *
diff --git a/includes/Modules/Analytics_4.php b/includes/Modules/Analytics_4.php
index 309a02fc300..988db9f9daf 100644
--- a/includes/Modules/Analytics_4.php
+++ b/includes/Modules/Analytics_4.php
@@ -93,6 +93,7 @@
 use Google\Site_Kit\Modules\Analytics_4\Conversion_Reporting\Conversion_Reporting_Cron;
 use Google\Site_Kit\Modules\Analytics_4\Conversion_Reporting\Conversion_Reporting_Events_Sync;
 use Google\Site_Kit\Modules\Analytics_4\Conversion_Reporting\Conversion_Reporting_Provider;
+use Google\Site_Kit\Modules\Analytics_4\Reset_Audiences;
 use stdClass;
 use WP_Error;
 
@@ -232,6 +233,8 @@ public function register() {
 
 		( new Advanced_Tracking( $this->context ) )->register();
 
+		( new Reset_Audiences( $this->user_options, $this ) )->register();
+
 		add_action( 'admin_init', array( $synchronize_property, 'maybe_schedule_synchronize_property' ) );
 		add_action( 'admin_init', array( $synchronize_adsense_linked, 'maybe_schedule_synchronize_adsense_linked' ) );
 		add_action( 'admin_init', array( $synchronize_ads_linked, 'maybe_schedule_synchronize_ads_linked' ) );
@@ -298,7 +301,6 @@ function ( $audience ) {
 							'adsLinked'                 => false,
 							'adsLinkedLastSyncedAt'     => 0,
 							'detectedEvents'            => array(),
-							'availableAudiencesLastSyncedAt' => 0,
 						)
 					);
 
diff --git a/includes/Modules/Analytics_4/Reset_Audiences.php b/includes/Modules/Analytics_4/Reset_Audiences.php
new file mode 100644
index 00000000000..cd76ec2175b
--- /dev/null
+++ b/includes/Modules/Analytics_4/Reset_Audiences.php
@@ -0,0 +1,175 @@
+<?php
+/**
+ * Class Google\Site_Kit\Modules\Analytics_4\Reset_Audiences
+ *
+ * @package   Google\Site_Kit\Modules\Analytics_4
+ * @copyright 2024 Google LLC
+ * @license   https://www.apache.org/licenses/LICENSE-2.0 Apache License 2.0
+ * @link      https://sitekit.withgoogle.com
+ */
+
+namespace Google\Site_Kit\Modules\Analytics_4;
+
+use Google\Site_Kit\Core\Dismissals\Dismissed_Items;
+use Google\Site_Kit\Core\Prompts\Dismissed_Prompts;
+use Google\Site_Kit\Core\Storage\User_Options;
+use Google\Site_Kit\Core\User\Audience_Settings;
+use Google\Site_Kit\Modules\Analytics_4;
+
+/**
+ * Class to reset Audience Segmentation Settings across multiple users.
+ *
+ * @since n.e.x.t
+ * @access private
+ * @ignore
+ */
+class Reset_Audiences {
+
+	/**
+	 * User_Options instance.
+	 *
+	 * @since n.e.x.t
+	 * @var User_Options
+	 */
+	protected $user_options;
+
+	/**
+	 * Dismissed_Prompts instance.
+	 *
+	 * @since n.e.x.t
+	 * @var Dismissed_Prompts
+	 */
+	protected $dismissed_prompts;
+
+	/**
+	 * Dismissed_Items instance.
+	 *
+	 * @since n.e.x.t
+	 * @var Dismissed_Items
+	 */
+	protected $dismissed_items;
+
+	/**
+	 * Analytics_4 instance.
+	 *
+	 * @since n.e.x.t
+	 * @var Analytics_4
+	 */
+	protected $analytics;
+
+	/**
+	 * Audience Settings instance.
+	 *
+	 * @since n.e.x.t
+	 * @var Audience_Settings
+	 */
+	protected $audience_settings;
+
+	const AUDIENCE_SEGMENTATION_DISMISSED_PROMPTS = array( 'audience_segmentation_setup_cta-notification' );
+
+	const AUDIENCE_SEGMENTATION_DISMISSED_ITEMS = array(
+		'audience-segmentation-add-group-notice',
+		'audience_segmentation_setup_success_notification',
+		'settings_visitor_groups_setup_success_notification',
+		'audience-segmentation-no-audiences-banner',
+		'audience-tile-*',
+	);
+
+	/**
+	 * Constructor.
+	 *
+	 * @since n.e.x.t
+	 *
+	 * @param User_Options $user_options User option API.
+	 * @param Analytics_4  $analytics    Analytics_4 instance.
+	 */
+	public function __construct( User_Options $user_options = null, Analytics_4 $analytics ) {
+		$this->user_options      = $user_options;
+		$this->dismissed_prompts = new Dismissed_Prompts( $this->user_options );
+		$this->dismissed_items   = new Dismissed_Items( $this->user_options );
+		$this->audience_settings = new Audience_Settings( $this->user_options );
+		$this->analytics         = $analytics;
+	}
+
+	/**
+	 * Register on change actions.
+	 *
+	 * @since n.e.x.t
+	 */
+	public function register() {
+		$this->analytics->get_settings()->on_change(
+			function ( $old_value, $new_value ) {
+				// Reset Audience specific settings, only when the Analytics propertyID changes.
+				if ( $old_value['propertyID'] !== $new_value['propertyID'] ) {
+					$this->reset_audience_data();
+				}
+			}
+		);
+	}
+
+	/**
+	 * Reset Audience specific settings for all SK users when propertyID changes.
+	 *
+	 * @since n.e.x.t
+	 */
+	public function reset_audience_data() {
+		global $wpdb;
+
+		// phpcs:ignore WordPress.DB.DirectDatabaseQuery
+		$users = $wpdb->get_col(
+			$wpdb->prepare(
+				"SELECT DISTINCT user_id
+				FROM $wpdb->usermeta
+				WHERE meta_key IN (%s, %s)
+				LIMIT 100 -- Arbitrary limit to avoid unbounded user iteration.",
+				$this->user_options->get_meta_key( Dismissed_Items::OPTION ),
+				$this->user_options->get_meta_key( Dismissed_Prompts::OPTION ),
+			)
+		);
+
+		if ( $users ) {
+			$backup_user_id = $this->user_options->get_user_id();
+
+			foreach ( $users as $user_id ) {
+				$this->user_options->switch_user( $user_id );
+
+				// Remove Audience Segmentation specific dismissed prompts.
+				foreach ( self::AUDIENCE_SEGMENTATION_DISMISSED_PROMPTS as $prompt ) {
+					$this->dismissed_prompts->remove( $prompt );
+				}
+
+				// Remove Audience Segmentation specific dismissed items.
+				foreach ( self::AUDIENCE_SEGMENTATION_DISMISSED_ITEMS as $item ) {
+					// Support wildcard matches, in order to delete all dismissed items prefixed with audience-tile-*.
+					if ( strpos( $item, '*' ) !== false ) {
+						$dismissed_items = $this->dismissed_items->get();
+
+						foreach ( array_keys( $dismissed_items ) as $existing_item ) {
+							if ( str_starts_with( $existing_item, rtrim( $item, '*' ) ) ) {
+								$this->dismissed_items->remove( $existing_item );
+							}
+						}
+					} else {
+						// For non-wildcard items, remove them directly.
+						$this->dismissed_items->remove( $item );
+					}
+				}
+
+				// Reset the users Audience Settings, such as configured audiences.
+				$this->audience_settings->reset();
+			}
+
+			// Restore original user.
+			$this->user_options->switch_user( $backup_user_id );
+		}
+
+		// Reset the main Analytics Module, Audience Segmentation settings.
+		$this->analytics->get_settings()->merge(
+			array(
+				'availableAudiences'                => null,
+				'availableAudiencesLastSyncedAt'    => 0,
+				'audienceSegmentationSetupComplete' => false,
+			)
+		);
+	}
+}
diff --git a/tests/phpunit/integration/Modules/Analytics_4/Reset_AudiencesTest.php b/tests/phpunit/integration/Modules/Analytics_4/Reset_AudiencesTest.php
new file mode 100644
index 00000000000..69bfc5bedbb
--- /dev/null
+++ b/tests/phpunit/integration/Modules/Analytics_4/Reset_AudiencesTest.php
@@ -0,0 +1,311 @@
+<?php
+/**
+ * Class Google\Site_Kit\Tests\Modules\Analytics_4\Reset_AudiencesTest
+ *
+ * @package   Google\Site_Kit\Tests\Modules\Analytics_4
+ * @copyright 2024 Google LLC
+ * @license   https://www.apache.org/licenses/LICENSE-2.0 Apache License 2.0
+ * @link      https://sitekit.withgoogle.com
+ */
+
+namespace Google\Site_Kit\Tests\Modules\Analytics_4;
+
+use Google\Site_Kit\Context;
+use Google\Site_Kit\Core\Authentication\Authentication;
+use Google\Site_Kit\Core\Dismissals\Dismissed_Items;
+use Google\Site_Kit\Core\Prompts\Dismissed_Prompts;
+use Google\Site_Kit\Core\Storage\Options;
+use Google\Site_Kit\Core\Storage\User_Options;
+use Google\Site_Kit\Core\User\Audience_Settings;
+use Google\Site_Kit\Modules\Analytics_4;
+use Google\Site_Kit\Modules\Analytics_4\Reset_Audiences;
+use Google\Site_Kit\Tests\TestCase;
+
+/**
+ * Class Google\Site_Kit\Tests\Modules\Analytics_4\Reset_AudiencesTest
+ *
+ * @group Modules
+ * @group Analytics
+ */
+class Reset_AudiencesTest extends TestCase {
+
+		/**
+	 * Context object.
+	 *
+	 * @var Context
+	 */
+	private $context;
+
+	/**
+	 * Options object.
+	 *
+	 * @var Options
+	 */
+	private $options;
+
+	/**
+	 * Array of User objects.
+	 *
+	 * @var array
+	 */
+	private $users;
+
+	/**
+	 * User Options object.
+	 *
+	 * @var User_Options
+	 */
+	private $user_options;
+
+	/**
+	 * Dismissed_Prompts instance.
+	 *
+	 * @since n.e.x.t
+	 * @var Dismissed_Prompts
+	 */
+	protected $dismissed_prompts;
+
+	/**
+	 * Dismissed_Items instance.
+	 *
+	 * @since n.e.x.t
+	 * @var Dismissed_Items
+	 */
+	protected $dismissed_items;
+
+	/**
+	 * Authentication object.
+	 *
+	 * @var Authentication
+	 */
+	private $authentication;
+
+	/**
+	 * Analytics 4 object.
+	 *
+	 * @var Analytics_4
+	 */
+	private $analytics;
+
+	/**
+	 * Audience Settings instance.
+	 *
+	 * @since n.e.x.t
+	 * @var Audience_Settings
+	 */
+	protected $audience_settings;
+
+	public function set_up() {
+		parent::set_up();
+
+		$this->context           = new Context( GOOGLESITEKIT_PLUGIN_MAIN_FILE );
+		$this->options           = new Options( $this->context );
+		$this->users             = array( $this->factory()->user->create_and_get( array( 'role' => 'administrator' ) ), $this->factory()->user->create_and_get( array( 'role' => 'editor' ) ) );
+		$this->user_options      = new User_Options( $this->context, $this->users[0]->ID );
+		$this->dismissed_prompts = new Dismissed_Prompts( $this->user_options );
+		$this->dismissed_items   = new Dismissed_Items( $this->user_options );
+		$this->audience_settings = new Audience_Settings( $this->user_options );
+		$this->authentication    = new Authentication( $this->context, $this->options, $this->user_options );
+		$this->analytics         = new Analytics_4( $this->context, $this->options, $this->user_options, $this->authentication );
+
+		$this->analytics->register();
+		wp_set_current_user( $this->users[0]->ID );
+
+		$this->analytics->get_settings()->merge(
+			array(
+				'propertyID' => 'UA-111111',
+			),
+		);
+
+		// Give each user a Dismissed Prompt so the reset code will run for each user.
+		$backup_user_id = $this->user_options->get_user_id();
+		foreach ( $this->users as $user ) {
+			$this->user_options->switch_user( $user->ID );
+			$this->dismissed_prompts->add( Reset_Audiences::AUDIENCE_SEGMENTATION_DISMISSED_PROMPTS[0] );
+		}
+		$this->user_options->switch_user( $backup_user_id );
+	}
+
+	public function test_reset_audience_segmentation_dismissed_prompts_across_multiple_users() {
+
+		$backup_user_id = $this->user_options->get_user_id();
+		foreach ( $this->users as $user ) {
+			$this->user_options->switch_user( $user->ID );
+			foreach ( Reset_Audiences::AUDIENCE_SEGMENTATION_DISMISSED_PROMPTS as $dismissed_prompt ) {
+				$this->dismissed_prompts->add( $dismissed_prompt );
+			}
+
+			$user_dismissed_prompts = $this->dismissed_prompts->get();
+			foreach ( Reset_Audiences::AUDIENCE_SEGMENTATION_DISMISSED_PROMPTS as $dismissed_prompt ) {
+				$this->assertTrue( array_key_exists( $dismissed_prompt, $user_dismissed_prompts ) );
+			}
+		}
+		$this->user_options->switch_user( $backup_user_id );
+
+		// Update the propertyID to trigger reset.
+		$this->analytics->get_settings()->merge(
+			array(
+				'propertyID' => 'UA-222222',
+			)
+		);
+
+		// Confirm the users dismissed items have been reset.
+		foreach ( $this->users as $user ) {
+			$this->user_options->switch_user( $user->ID );
+
+			$user_dismissed_prompts = $this->dismissed_prompts->get();
+			foreach ( Reset_Audiences::AUDIENCE_SEGMENTATION_DISMISSED_PROMPTS as $dismissed_prompt ) {
+				$this->assertFalse( array_key_exists( $dismissed_prompt, $user_dismissed_prompts ) );
+			}
+		}
+		$this->user_options->switch_user( $backup_user_id );
+	}
+
+	public function test_reset_audience_segmentation_dismissed_items_across_multiple_users() {
+
+		$test_dismissed_items = array_merge(
+			Reset_Audiences::AUDIENCE_SEGMENTATION_DISMISSED_ITEMS,
+			array(
+				'audience-tile-testAudienceTile1',
+				'audience-tile-testAudienceTile2',
+			)
+		);
+		// Remove the wildcard key, as we have added test examples above.
+		unset( $test_dismissed_items[ array_search( 'audience-tile-*', $test_dismissed_items, true ) ] );
+
+		// Set possible dismissed items for each user.
+		$backup_user_id = $this->user_options->get_user_id();
+		foreach ( $this->users as $user ) {
+			$this->user_options->switch_user( $user->ID );
+
+			foreach ( $test_dismissed_items as $dismissed_item ) {
+				$this->dismissed_items->add( $dismissed_item );
+			}
+
+			$user_dismissed_items = $this->dismissed_items->get();
+			foreach ( $test_dismissed_items as $dismissed_item ) {
+				$this->assertTrue( array_key_exists( $dismissed_item, $user_dismissed_items ) );
+			}
+		}
+		$this->user_options->switch_user( $backup_user_id );
+
+		// Update the propertyID to trigger reset.
+		$this->analytics->get_settings()->merge(
+			array(
+				'propertyID' => 'UA-222222',
+			)
+		);
+
+		// Confirm the users dismissed items have been reset.
+		foreach ( $this->users as $user ) {
+			$this->user_options->switch_user( $user->ID );
+
+			$user_dismissed_items = $this->dismissed_items->get();
+			foreach ( $test_dismissed_items as $dismissed_item ) {
+				$this->assertFalse( array_key_exists( $dismissed_item, $user_dismissed_items ) );
+			}
+		}
+		$this->user_options->switch_user( $backup_user_id );
+	}
+
+	public function test_reset_audience_segmentation_configured_audience_settings_across_multiple_users() {
+
+		$default_user_audience_settings = array(
+			'configuredAudiences'                => null,
+			'isAudienceSegmentationWidgetHidden' => false,
+			'didSetAudiences'                    => false,
+		);
+
+		$activated_user_audience_settings = array(
+			'configuredAudiences'                => array(
+				'properties/12345678/audiences/12345',
+				'properties/12345678/audiences/67890',
+			),
+			'isAudienceSegmentationWidgetHidden' => true,
+			'didSetAudiences'                    => true,
+		);
+
+		// Set configured audiences for each user.
+		$backup_user_id = $this->user_options->get_user_id();
+		foreach ( $this->users as $user ) {
+			$this->user_options->switch_user( $user->ID );
+
+			$this->audience_settings->merge(
+				array(
+					'configuredAudiences'                => $activated_user_audience_settings['configuredAudiences'],
+					'isAudienceSegmentationWidgetHidden' => $activated_user_audience_settings['isAudienceSegmentationWidgetHidden'],
+				)
+			);
+
+			$audience_settings = $this->audience_settings->get();
+			foreach ( array_keys( $default_user_audience_settings ) as $key ) {
+				$this->assertEquals( $activated_user_audience_settings[ $key ], $audience_settings[ $key ] );
+			}
+		}
+		$this->user_options->switch_user( $backup_user_id );
+
+		// Update the propertyID to trigger reset.
+		$this->analytics->get_settings()->merge(
+			array(
+				'propertyID' => 'UA-222222',
+			)
+		);
+
+		// Confirm the users audience settings have been reset.
+		foreach ( $this->users as $user ) {
+			$this->user_options->switch_user( $user->ID );
+			$audience_settings = $this->audience_settings->get();
+			foreach ( array_keys( $default_user_audience_settings ) as $key ) {
+				// As 'configuredAudiences' is updated to null, this key is removed.
+				if ( 'configuredAudiences' === $key ) {
+					$this->assertFalse( array_key_exists( $key, $audience_settings ) );
+				} else {
+					$this->assertEquals( $default_user_audience_settings[ $key ], $audience_settings[ $key ] );
+				}
+			}
+		}
+		$this->user_options->switch_user( $backup_user_id );
+	}
+
+	public function test_reset_audience_segmentation_module_settings() {
+		$default_audience_segmentation_settings = array(
+			'availableAudiences'                => null,
+			'availableAudiencesLastSyncedAt'    => 0,
+			'audienceSegmentationSetupComplete' => false,
+		);
+
+		$activated_audience_segmentation_settings = array(
+			'availableAudiences'                => array(
+				array(
+					'name' => 'properties/12345678/audiences/12345',
+				),
+				array(
+					'name' => 'properties/12345678/audiences/67890',
+				),
+			),
+			'availableAudiencesLastSyncedAt'    => time(),
+			'audienceSegmentationSetupComplete' => true,
+		);
+
+		$this->analytics->get_settings()->merge(
+			$activated_audience_segmentation_settings
+		);
+
+		$analytics_settings = $this->analytics->get_settings()->get();
+		foreach ( array_keys( $default_audience_segmentation_settings ) as $key ) {
+			$this->assertEquals( $activated_audience_segmentation_settings[ $key ], $analytics_settings[ $key ] );
+		}
+
+		// Update the propertyID to trigger reset.
+		$this->analytics->get_settings()->merge(
+			array(
+				'propertyID' => 'UA-222222',
+			)
+		);
+
+		$analytics_settings = $this->analytics->get_settings()->get();
+		foreach ( array_keys( $default_audience_segmentation_settings ) as $key ) {
+			$this->assertEquals( $default_audience_segmentation_settings[ $key ], $analytics_settings[ $key ] );
+		}
+	}
+}