diff --git a/python/tests/test_highlevel.py b/python/tests/test_highlevel.py
index b124ea6fca..9d169abeed 100644
--- a/python/tests/test_highlevel.py
+++ b/python/tests/test_highlevel.py
@@ -3867,6 +3867,21 @@ def test_branch_length_empty_tree(self):
         assert tree.branch_length(1) == 0
         assert tree.total_branch_length == 0
 
+    @pytest.mark.parametrize("root_threshold", [1, 2, 3])
+    def test_is_root(self, root_threshold):
+        # Make a tree with multiple roots with different numbers of samples under each
+        ts = tskit.Tree.generate_balanced(5).tree_sequence
+        ts = ts.decapitate(ts.max_root_time - 0.1)
+        tables = ts.dump_tables()
+        tables.nodes.add_row(flags=0)  # Isolated non-sample
+        tables.nodes.add_row(flags=tskit.NODE_IS_SAMPLE)  # Isolated sample
+        ts = tables.tree_sequence()
+        assert {ts.first().num_samples(u) for u in ts.first().roots} == {1, 2, 3}
+        tree = ts.first(root_threshold=root_threshold)
+        roots = set(tree.roots)
+        for u in range(ts.num_nodes):  # Will also test isolated nodes
+            assert tree.is_root(u) == (u in roots)
+
     def test_is_descendant(self):
         def is_descendant(tree, u, v):
             path = []
diff --git a/python/tskit/trees.py b/python/tskit/trees.py
index 23cf681b4b..c3519cf86a 100644
--- a/python/tskit/trees.py
+++ b/python/tskit/trees.py
@@ -1574,6 +1574,21 @@ def root(self):
             raise ValueError("More than one root exists. Use tree.roots instead")
         return self.left_root
 
+    def is_root(self, u) -> bool:
+        """
+        Returns ``True`` if the specified node is a root in this tree. See
+        :attr:`~Tree.roots` for the definition of a root. Note that this will return
+        ``False`` for all other nodes, including
+        :ref:`isolated<sec_data_model_tree_isolated_nodes>` non-sample nodes which are
+        not attached to the topology of the current tree.
+
+        :param int u: The node of interest.
+        :return: ``True`` if u is a root.
+        """
+        return (
+            self.num_samples(u) >= self.root_threshold and self.parent(u) == tskit.NULL
+        )
+
     def get_index(self):
         # Deprecated alias for self.index
         return self.index