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shift read correction for ATAC/Cut&Tag #1056

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sunta3iouxos opened this issue Sep 10, 2024 · 0 comments
Open

shift read correction for ATAC/Cut&Tag #1056

sunta3iouxos opened this issue Sep 10, 2024 · 0 comments

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@sunta3iouxos
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Lot's of meta applications of ATAC or Cut&Tag require a correction/Shift read coordinates, introdused by Tn5.
A good tool to do so and can be implemented in the pipeline is this, and it is a deeptool:
alignmentSieve --numberOfProcessors max --ATACshift --blackListFileName hg38-blacklist.v2.bed --bam blacklist-filtered.bam -o ${prefix}.shifted.bam
Is this implemented?
In general for ATAC seq you need one track/bw for visualisation and another for footprint/motif discovery etc.

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