The format is based on Keep a Changelog and this project adheres to Semantic Versioning.
Update the pipeline to nf-core 2.5.1, update modules, and fix documentation.
- Documentation: Correct aligner option documentation
- Update
samplesheet_check
module - Update
dumpsoftwareversion
module - Update
MultiQC
module
Improves computation time.
Split uLTRA pipeline
into two processes, uLTRA index
and uLTRA align
. GTF
index is computed once and not chunk
times.
uLTRA align
sort and convert sam
output into bam
files. Aligned reads are already sorted by minimap2
module. Therefore, samtools sort
module is not needed anymore and has been removed.
The bioperl
module objective was to deal with spurious alignments produced by uLTRA if a malformed GTF is used. Removing it will stop the pipeline in case of malformed GTF
.
Module resource requirements have been revised for four modules (gstama/merge
, isoseq3/refine
, lima
, ultra/align
) to reduce requested resources.
AWS runs with shows better run time and CPU/RAM usage (Results).
- Add
uLTRA index
anduLTRA align
to replaceuLTRA pipeline
PR 1830 - Module resources adjustments:
gstama/merge
,isoseq3/refine
,lima
,ultra/align
PR1858,gunzip
,MultiQC
- Remove
uLTRA pipeline
- Remove
samtools sort
module - Remove
bioperl
module
Initial release of nf-core/isoseq, created with the nf-core template.