diff --git a/.changeset/yellow-insects-tell.md b/.changeset/yellow-insects-tell.md
new file mode 100644
index 000000000000..576ae3a18213
--- /dev/null
+++ b/.changeset/yellow-insects-tell.md
@@ -0,0 +1,5 @@
+---
+'astro': patch
+---
+
+Fix: ignore `.json` files nested in subdirectories within content collection directories starting with an `_` underscore.
diff --git a/packages/astro/src/content/vite-plugin-content-virtual-mod.ts b/packages/astro/src/content/vite-plugin-content-virtual-mod.ts
index 021a26314b72..d7924973cc24 100644
--- a/packages/astro/src/content/vite-plugin-content-virtual-mod.ts
+++ b/packages/astro/src/content/vite-plugin-content-virtual-mod.ts
@@ -213,7 +213,7 @@ function globWithUnderscoresIgnored(relContentDir: string, exts: string[]): stri
 	const contentDir = appendForwardSlash(relContentDir);
 	return [
 		`${contentDir}**/*${extGlob}`,
-		`!${contentDir}**/_*/**${extGlob}`,
+		`!${contentDir}**/_*/**/*${extGlob}`,
 		`!${contentDir}**/_*${extGlob}`,
 	];
 }
diff --git a/packages/astro/test/fixtures/content-collections/src/content/with-schema-config/_ignore/ignore/file.md b/packages/astro/test/fixtures/content-collections/src/content/with-schema-config/_ignore/ignore/file.md
new file mode 100644
index 000000000000..e69de29bb2d1