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[BUG] Harmony pipelines does not give reproducible results #210

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dweemx opened this issue May 12, 2020 · 1 comment · Fixed by #213 or vib-singlecell-nf/harmony#10
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[BUG] Harmony pipelines does not give reproducible results #210

dweemx opened this issue May 12, 2020 · 1 comment · Fixed by #213 or vib-singlecell-nf/harmony#10
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@dweemx
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dweemx commented May 12, 2020

Describe the bug
The *harmony* pipelines does not give reproducible results in terms of cell embeddings of PCA, t-SNE and UMAP.

To Reproduce
Steps to reproduce the behavior:

  1. Configure with these options:
nextflow config vib-singlecell-nf/vsn-pipelines -profile tenx,pcacv,harmony,singularity > nextflow.config
...
  1. Run using this entry point:
nextflow -C nextflow.config run vib-singlecell-nf/vsn-pipelines -entry harmony
  1. See error:

/ (No explicit errors)

Expected behavior
The cell embeddings of PCA, t-SNE and UMAP should correspond between 2 or more independent runs given the same seed.

Screenshots
Run 1:
image

Run 2:
image

Please complete the following information:

  • OS: CentOS 7
  • Nextflow Version: 19.12.0-edge
  • vsn-pipelines Version: v0.18.0

Additional context
Related to #165

@dweemx dweemx added the bug Something isn't working label May 12, 2020
@dweemx dweemx self-assigned this May 12, 2020
dweemx added a commit to vib-singlecell-nf/harmony that referenced this issue May 13, 2020
@dweemx
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dweemx commented May 13, 2020

Using params.global.seed = 180, we should get the following results:
image

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